Identification and Functional Analysis of Betv1 in Three D Genome-containing Wild Cotton Varieties

Abstract:

[Objective] By analyzing the major birch pollen allergen Betv1 gene family in D genomes of cotton and comparing the expression patterns of three diploid D-genome cotton varieties with different Verticillium wilt resistance levels, we aimed to provide a theoretical basis for further studies on the role of Betv1 genes in cotton resistant to Verticillium wilt. [Method] The Betv1 genes were identified, and a bioinformatics analysis of the physicochemical properties of their encoded sequences in Gossypium raimondii (D5) was performed. The transcriptome sequencing and quantitative real time-PCR of G. raimondii (D5), Gossypium trilobum (D8) and Gossypium thurberi (D1) were used to verify the expression patterns of Bet v 1 genes under Verticillium dahlia infection stress. Betv1 genes were silenced by virus-induced gene silencing in G. hirsutum to identify their functions. [Result] The D genome of cotton contains 59 members, 58 of which have introns and are distributed on eight chromosomes, and most encode hydrophilic proteins that localize to the cytoplasm. The expression levels of Betv1 genes in three wild cotton species having D genomes after being inoculated with V. dahliae were consistent with their disease resistance levels. The genes were separated into three groups based on their expression levels. Genes of Group 3 responded to V. dahliae infection and were highly expressed in disease-resistant cotton species G. thurberi. This indicated that Group 3 genes may be involved in the immune response of Verticillium wilt. A gene with a high expression level was screened out of Group 3. A corresponding homologous gene was silenced in G. hirsutum by virus-induced gene silencing, and gene-silenced plants were more susceptible to V. dahliae, indicating that the gene plays a positive regulatory role in the progress of Verticillium wilt resistance in cotton. [Conclusion] The Betv1 genes act in response to V. dahliae infection and are critical in cotton resistance to Verticillium wilt. The information obtained provides a basis for further studies of the cotton Bet v 1 family genes and their functions.

Key words: wild cotton; Verticillium wilt; Betv1 gene; transcriptome; quantitative real time-PCR (qRT-PCR); virus-induced gene silencing (VIGS)

Cotton Science. 2019, 31(5):361-380.

https://doi.org/10.11963/1002-7807.dqmzy.20190723

World Cotton Day

07 October 2019 has been announced as World Cotton Day by WTO. Cotton community will celebrate this day across the globe. The WTO Secretariat is organizing the event at WTO Headquarters Geneva in collaboration with the Secretariats of the United Nations Food and Agriculture Organization (FAO), the United Nations Conference on Trade and Development (UNCTAD), the International Trade Centre (ITC) and the International Cotton Advisory Committee (ICAC). This event stems from the Cotton-4’s official application for the recognition of a World Cotton Day by the United Nations General Assembly, reflecting the importance of cotton as a global commodity.

For detail

Isolation and characterization of the GbVIP1gene and response to Verticillium wilt in cotton and tobacco

Isolation and characterization of the GbVIP1gene and response to Verticillium wilt in cotton and tobacco

  • ZHANG Kai ,
  • ZHAO Pei ,
  • WANG Hongmei Email author,
  • ZHAO Yunlei ,
  • CHEN Wei ,
  • GONG Haiyan ,
  • SANG Xiaohui  and
  • CUI Yanli 
Contributed equally
Journal of Cotton Research20192:2

https://doi.org/10.1186/s42397-019-0019-0

Abstract

Background

Verticillium wilt is a serious soil-borne vascular disease that causes major losses to upland cotton (Gossypium hirutum L.) worldwidely every year. The protein VIP1 (VirE2 interaction protein 1), a bZIP transcription factor, is involved in plant response to many stress conditions, especially pathogenic bacteria. However, its roles in cotton response to Verticillium wilt are poorly understood.

Results

The GbVIP1 gene was cloned from resistant sea-island cotton (G. barbadense) cv. Hai 7124. Expression of GbVIP1 was up-regulated by inoculation with Verticillium dahliae and exogenous treatment with ethylene. Results of virus-induced gene silencing suggested that silencing of GbVIP1 weakened cotton resistance to Verticillium wilt. The heterologous expression of GbVIP1 in tobacco showed enhanced resistance to Verticillium wilt. The PR1, PR1-like and HSP70 genes were up-regulated in GbVIP1 transgenic tobacco after Verticillium wilt infection.

Conclusion

Our results suggested that GbVIP1 increased plant resistance to Verticillium wilt through up-regulating expressions of PR1, PR1-like, and HSP70. These results provide new approaches to improving resistance to Verticillium wilt in upland cotton and also have great potential for disease-resistance breeding of cotton.

Keywords

  • Cotton
  • VIP1
  • Verticillium wilt

https://jcottonres.biomedcentral.com/articles/10.1186/s42397-019-0019-0

Management of Insecticide Resistant Helicoverpa armigera (Lepidoptera: Noctuidae) in Cotton in India

ABSTRACT
Helicoverpa armigera (Hübner) was responsible for some $500 million of damage in India in the 1997-8. Insecticide resistance levels have been monitored routinely at sites throughout the country since 1992 using discriminating dose assays. Resistance to pyrethroids is ubiquitous and stable at around 50-80% in most areas. Organophosphate and endosulfan resistance is stable at around 20-50%. Carbamate resistance is low. There is currently no significant resistance to Bt. Putting in place effective, economic, insecticide-based programmes that do not exacerbate the resistance problems, is a priority. Field trials from 1992-5 at ICRISAT developed appropriate IPM/IRM practices in pigeonpea and cotton. In 1995-6 these were taken into ‘split-plot’ IPM/IRM farmer trials in Andhra Pradesh (AP). Seed cotton yields were slightly enhanced with a 23% reduction in the number of insecticide sprays and a 57% reduction in the a.i. applied. In expanded trials in 1996-7 trials in AP and Tamil Nadu, insecticide use was reduced by over 40% and yields enhanced by 30%. Components of the package included appropriate varietal selection, seed quality and agronomy and improved spraying practices with quality materials based on scouting to simple economic thresholds. Early season spraying for sucking pests was avoided by the use of systemic seed dressings. The sequence used for bollworms was: Eggs: at low numbers – neem; at high numbers – profenofos, Larvae: 1st round: endosulfan; 2nd round OPs (quinalphos or chlorpyrifos); 3rd round, carbamates (carbaryl); 4th round – pyrethroids (cypermethrin, fenvalerate, deltamethrin or lambda cyhalothrin). In the 1997-8 season farmer participatory demonstrations were undertaken in four states (Punjab, Andhra Pradesh, Maharashtra and Tamil Nadu). In all areas spray applications were at least halved with respect to non-participating farmers and yields rose by at least a third. The work was expanded in the 1998-9 season.

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JCR-Functional Genomics and Biotechnology Thematic Series Call for Paper

Journal of Cotton Research

Functional Genomics and Biotechnology

Thematic Series Call for Paper

Coordinators:
Professor Fuguang LI, Institute of Cotton Research, CAAS, China
Dr. Zuoren YANG, Institute of Cotton Research, CAAS, China

Remarkable advances have been made in cotton functional genomics and biotechnology, though more findings are expected in most fields in functional genomics. Journal of Cotton Research is hosting a thematic series on Functional genomics and biotechnology. The research community is encouraged to share original findings, methodology, results, databases, and/or software and opinions.

Scopes that may be covered in the submissions may include, but are not limited to the following:

1. Quantitative trait loci/genes related to fiber quality, yield or stress-resistance from different Gossypium species;
2. Gene regulatory network;
3. Genome editing (Focusing on CRISPR/Cas9 system);
4. Bioinformatics: Tools, software and database, etc;
5. Cotton genetic improvement.

Submission Deadline: 31 August 2019

https://jcottonres.biomedcentral.com/cottonfgb

JCR-Cotton High Speed Phenotyping Thematic Series Call For Paper

Journal of Cotton Research

Cotton High Speed Phenotyping

Thematic Series Call For Paper

Coordinator: Professor Eric F. Hequet, Texas Tech University, USA; Dr. Glen Ritchie, Texas Tech University, USA

High speed phenotyping is critical to improve cotton research and production. It can be applied to large scale commercial fields, research fields, breeding lines, and even at the individual plant level. The main goals are to improve yield, fiber quality, stress and disease resistance, etc… Recently, advances in high speed phenotyping in cotton have been achieved. The Journal of Cotton Research is hosting a thematic series on this topic. The research community is encouraged to share original findings, methodology, results, databases, and/or software and opinions.

Scopes that may be covered in the submissions may include, but are not limited to the following:
1. Platform design: air-based and/or land-based;
2. Data capture and processing: sensors (RGB, IR, multispectral, sonic, etc.), integration of multiple sensors, information processing technologies;
3. Data analysis and Metadata: analysis of very large data sets, validation with ground truth, practical application examples (breeding programs, site specific irrigation scheduling, etc.).

Submission Deadline: April 30, 2019

https://jcottonres.biomedcentral.com/cottonhsp

Administrative Control of PCCC

The administrative control of Pakistan Central Cotton Committee (PCCC) has been shifted from Ministry of Commerce and Textile to the Ministry of National Food Security and Research. The decision is taken by the Government keeping in view the suggestions made by a high level review committee. The decision is hoped to favorably impact cotton research and development activities at national level.

Technical Assistance for C-4 Countries

A total of 7 delegates from Burkina Faso, Chad, Mali and Benin will be visiting Pakistan from April 22 to May 05, 2018. During the stay in Pakistan delegates will make exposure visits of different cotton research Institutions, textile and garment industry  and academic institutions at Lahore, Faisalabad and Multan. A full day is reserved for Pakistan Central Cotton Committee and Central Cotton Research Institute at Multan. The visit will be coordinated by Dr. Khalid Abdullah Cotton Commissioner and Vice President PCCC.

The training is offered by Government of Pakistan under the “Technical Assistance Program for C-4 Countries”

ICRA Secretariat goes functional

Signature Ceremony in Multan

The MOU between ICRA and PCCC has been signed on October 9, 2017, in Multan, in the framework of a ceremony organized by PCCC to which attended around thirty participants coming from research institutes and agricultural universities.

Dr. Michel FOK, ICRA Chair visited Pakistan from 09-12.10.2017 for the MOU signing ceremony. PCCC has nominated Dr. Fiaz Ahmad, Senior Scientist of Central Cotton Research Institute, to serve  as Secretary, assisted by Mr. Zahid Khan.

Dr. FOK visited cotton trials at Research Institutes, Research Labs, two agricultural Universities of Multan. He visited cotton fields of two Progressive farmers in the surroundings of Multan. He gave two lectures about the concept of climate smart agriculture and the Chinese experience in using Bt-cotton. MOU ceremony

ICRA Office (Outer View)

Office-Outer

ICRA Office (Inner-View)

Office-Inner

ICRA Committee Room

Committee Room

PCCC Building

ICRA Group