An isopentyl transferase gene driven by the senescence-inducible SAG12 promoter improves salinity stress tolerance in cotton

Journal of Cotton Research

[Background] Soil salinity seriously affects cotton growth, leading to the reduction of yield and fiber quality. Recently, genetic engineering has become an efficient tool to increase abiotic stress tolerance in crops.

[Results] In this study, isopentyl transferase (IPT), a key enzyme involved in cytokinin (CTK) biosynthesis from Agrobacterium tumefaciens, was selected to generate transgenic cotton via Agrobacterium-mediated transformation. A senescence-inducible SAG12promoter from Arabidopsis was fused with the IPT gene. Ectopic-expression of SAG12::IPT significantly promoted seed germination or seedling tolerance to salt stress. Two IPTtransgenic lines, OE3 as a tolerant line during seed germination, and OE8 as a tolerant line at seedling stage, were selected for further physiological analysis. The data showed that ectopic-expression of SAG12::IPT induced the accumulation of CTKs not only in leaves and roots, but also in germinating seeds. Moreover, ectopic-expressing IPT increased the activity of antioxidant enzymes, which was associated with the less reactive oxygen species (ROS) accumulation compared with control plants. Also, ectopic-expression of IPT produced higher K+/Na+ ratio in cotton shoot and root.

[Conclusions] The senescence-induced CTK accumulation in cotton seeds and seedlings positively regulates salt stress partially by elevating ROS scavenging capability.

[TitleAn isopentyl transferase gene driven by the senescence-inducible SAG12 promoter improves salinity stress tolerance in cotton

[Authors] SHAN Yi, ZHAO Peng, LIU Zhao, LI Fangjun* & TIAN Xiaoli

Journal of Cotton Research. 2019, 2: 15

JCR-Cotton High Speed Phenotyping Thematic Series Call For Paper

Journal of Cotton Research

Cotton High Speed Phenotyping
Thematic Series Call For Paper
Coordinator: Professor Eric F. Hequet, Texas Tech University, USA; Dr. Glen Ritchie, Texas Tech University, USA

Author’s allowance: The sponsor, Institute of Cotton Research, CAAS, grants to cover not only APC for the submission, but also the author’s allowance once published.

High speed phenotyping is critical to improve cotton research and production. It can be applied to large scale commercial fields, research fields, breeding lines, and even at the individual plant level. The main goals are to improve yield, fiber quality, stress and disease resistance, etc… Recently, advances in high speed phenotyping in cotton have been achieved. The Journal of Cotton Research is hosting a thematic series on this topic. The research community is encouraged to share original findings, methodology, results, databases, and/or software and opinions.

Scopes that may be covered in the submissions may include, but are not limited to the following:
1. Platform design: air-based and/or land-based;
2. Data capture and processing: sensors (RGB, IR, multispectral, sonic, etc.), integration of multiple sensors, information processing technologies;
3. Data analysis and Metadata: analysis of very large data sets, validation with ground truth, practical application examples (breeding programs, site specific irrigation scheduling, etc.).

Submission Deadline: April 30, 2019

Cotton-innovation website

Cotton-innovation website:

A site dedicated to cotton innovations for West and Central Africa, an English version of this website is now available

Factsheets are available on innovations in the cotton supply chains, sustainability indicators for cotton farming systems (related to the SEEP report), economic information, etc.

Enjoy your visit.


In a Nutshell: Cotton is Renewable, Recyclable and 100% Biodegradable 

PRESS RELEASE by Elke Hortmeyer, Director of Communications and International Relations, Bremen. 
Bremen, April 4th, 2017: With its attributes ‘renewable’, ‘recyclable’ and ‘biodegradable’, the Bremen Cotton Exchange sees cotton as one of the most sustainable raw materials on the planet from an ecological, social and economic point of view. 
Depending on the prevailing conditions, nature can degrade cotton residues in soil within six months, so that they are returned to the earth. The same applies to cotton fibres, which are passed into wastewater through the washing of clothes and textiles in private households and ultimately into rivers and seas. Because cotton is biodegradable, this cannot lead to any situations which endanger humans, animals or nature, as is often the case with the growing problem of microplastics in the sea. 
As an agricultural product, cotton is a renewable raw material that can be grown once or even twice, depending on the climate or region. Every part of the plant can be used: Valuable oils for the food and cosmetic industries can be produced from the seed grains. The cotton fibres are used to produce yarns and fabrics for home and household textiles, clothing, hygienic and medical products, or for their use in technical applications, e.g. lightweight construction. Non-spinnable short fibres, the linters, are processed by the paper industry, or even for spectacle frames, while animal feeds, products for soil improvement or packaging and insulation material are made from cotton residues.  Textile and clothing products made of cotton are 100% recyclable. They are sorted according to colour, enter the shredder, are crushed and can then be used as new yarns and fabrics for use in clothing and textiles. Another example of sensible recycling is the processing of cotton residues into a cellulose fibre. This is carried out in a manufacturing process similar to that of viscose. In this case, too, there is 100% biodegradability. 
We would be pleased to answer any further questions – also in an interview. 
Elke Hortmeyer, Director of Communications and International Relations 
Tel.:+49 421 3397016, Email: 3