Effects of NaCl stress on the biochemical substances in Bt cotton as well as on the growth and development and adult oviposition selectivity of Helicoverpa armigera

Background

Recently, due to the development of food security strategies, cotton has been planted in inland saline-alkali dry soils or in coastal some saline-alkali soils in China. Under the condition, to comprehensively prevent and control Helicoverpa armigera in cotton fields with saline-alkali soils, it is important to study the larval growth and development of H. armigera and to study adult oviposition selectivity in H. armigera adults that feed on NaCl-stressed cotton plants.

Results

In this study, Bt cotton GK19 was used for the experimental group and its nontransgenic parent Simian 3 was used for the control to study the effects of biochemical substances in cotton as well as larval growth and development and adult oviposition selectivity of H. armigera. The experiments were performed by growing cotton indoors under NaCl stress at concentrations of 0 mmol·L− 1, 75 mmol·L− 1 and 150 mmol·L− 1, respectively. The results showed that the expression of Bt protein was significantly inhibited for NaCl-stressed Bt cotton. The content of soluble protein and K+ in the leaves of cotton were decreased, while the content of gossypol and Na+ were increased. In addition, the 5th instar H. armigera larvae exhibited shorten the life span in a 13-day trial period. Under enclosure treatments and at different female densities, the adult oviposition of H. armigera decreased on high NaCl-stressed nontransgenic cotton, while the oviposition on Bt cotton tended to first increase but then decrease under low, moderate and high NaCl stress treatments.

Conclusions

Under certain content ranges of NaCl stress treatments, larval of H. armigera growth and development, and adult oviposition were no significant difference in the change for a certain period. However, under high NaCl stress, larval growth, development and adult oviposition were affected, which may provide insights for the prevention and control of H. armigera for Bt cotton in saline-alkali soils.

 

Effects of NaCl stress on the biochemical substances in Bt cotton as well as on the growth and development and adult oviposition selectivity of Helicoverpa armigera

LUO Junyu, ZHANG Shuai, ZHU Xiangzhen, JI Jichao, ZHANG Kaixin, WANG Chunyi, ZHANG Lijuan, WANG Li and CUI Jiniie

Journal of Cotton Research. 2019; 2:4

https://doi.org/10.1186/s42397-019-0020-7

 

https://jcottonres.biomedcentral.com/articles/10.1186/s42397-019-0020-7

JCR-Genome-wide identification of Gossypium INDETERMINATE DOMAIN genes and their expression profiles in ovule development and abiotic stress responses

Background

INDETERMINATE DOMAIN (IDD) transcription factors form one of the largest and most conserved gene families in plant kingdom and play important roles in various processes of plant growth and development, such as flower induction in term of flowering control. Till date, systematic and functional analysis of IDDgenes remained infancy in cotton.

Results

In this study, we identified total of 162 IDD genes from eight different plant species including 65 IDD genes in Gossypium hirsutum. Phylogenetic analysis divided IDDs genes into seven well distinct groups. The gene structures and conserved motifs of GhIDD genes depicted highly conserved exon-intron and protein motif distribution patterns. Gene duplication analysis revealed that among 142 orthologous gene pairs, 54 pairs have been derived by segmental duplication events and four pairs by tandem duplication events. Further, Ka/Ksvalues of most of orthologous/paralogous gene pairs were less than one suggested the purifying selection pressure during evolution. Spatiotemporal expression pattern by qRT-PCR revealed that most of the investigated GhIDD genes showed higher transcript levels in ovule of seven days post anthesis, and upregulated response under the treatments of multiple abiotic stresses.

Conclusions

Evolutionary analysis revealed that IDD gene family was highly conserved in plant during the rapid phase of evolution. Whole genome duplication, segmental as well as tandem duplication significantly contributed to the expansion of IDDgene family in upland cotton. Some distinct genes evolved into special subfamily and indicated potential role in the allotetraploidy Gossypium hisutum evolution and development. High transcript levels of GhIDD genes in ovules illustrated their potential roles in seed and fiber development. Further, upregulated responses of GhIDD genes under the treatments of various abiotic stresses suggested them as important genetic regulators to improve stress resistance in cotton breeding.

Genome-wide identification of Gossypium INDETERMINATE DOMAIN genes and their expression profiles in ovule development and abiotic stress responses

ALI Faiza, QANMBER Ghulam, LI Yonghui, MA Shuya, LU Lili, YANG Zuoren, WANG Zhi and LI Fuguang

Journal of Cotton Research 2019 2:3

https://jcottonres.biomedcentral.com/articles/10.1186/s42397-019-0021-6

JCR-Physiology and Pathology Thematic Series Call For Paper

Coordinator:
Professor Hezhong DONG, Cotton Research Centre, Shandong Academy of Agricultural Sciences, China;
Professor Zhiguo ZHOU, Nanjing Agricultural University, China

Journal of Cotton Research is hosting a thematic series on cotton physiology and pathology. The research community is encouraged to share original findings, methodology, results, databases, and/or software and opinions.

Scopes that may be covered in the submissions may include, but are not limited to the following:

1. growth and organ development;
2. plant and shoot architecture;
3. water, fertilizer, light, etc. use efficiency;
4. intelligent agriculture;
5. rhizosphere and other plant-environment interactions;
6. main disease and resistance.

Submission Deadline: 30 September 2019

Submit Now

https://jcottonres.biomedcentral.com/cottonpap

Evaluation of genetic diversity in short duration cotton (Gossypium hirsutum L.)

[Background] Cotton (Gossypium hirsutum L.) is an important fiber crop in Bangladesh. Genetic diversity among the genotypes of a germplasm has a great importance for cotton breeding. An experiment was carried out at the experimental field of Cotton Research, Training and Seed Multiplication Farm, Sreepur, Gazipur during the cropping season of 2015–2016 with 100 genotypes to evaluate genetic diversity of cotton genotypes for short duration using field performance.

[Results] The genotypes under study were grouped into ten clusters through multivariate analysis using GENSTAT-5. Cluster III contained maximum number of genotypes (16) while cluster X contained the least number of genotypes (7). The inter cluster distances were larger than intra cluster distances in all cases suggesting wider genetic diversity among the genotypes of different clusters. The maximum and minimum inter cluster distances were observed between clusters II and V (10.78) and clusters VIII and IX (3.30), respectively. The results indicated diverse and close relationship among the genotypes of those clusters. Earliness index, single boll weight and days to boll opening showed the higher contribution to the genetic divergence among 19 characters.

[Conclusion] Based on the results of genetic diversity and earliness index, the genotypes from cluster II could be used as parent in hybridization program for the development of short duration cotton variety.

Evaluation of genetic diversity in short duration cotton (Gossypium hirsutum L.)

AKTER T., ISLAM A. K. M. A., RASUL M. G., KUNDU S., KHALEQUZZAMAN and AHMED J. U.
Journal of Cotton Research. 2019; 2:1.
https://doi.org/10.1186/s42397-018-0018-6

Irrigation’s effect and applied selection on the fiber quality of Ethyl MethaneSulfonate (EMS) treated upland cotton (Gossypium hirsutum L.)

[Background] Producing rainfed cotton (Gossypium hirsutum L.) with high fiber quality has been challenging in the Texas High Plains because of extended periods of insufficient rainfall during sensitive boll developmental stages. Genetic variation created by Ethyl MethaneSulfonate (EMS) mutagen has successfully improved fiber quality of cotton. However, little is known about the effect of water deficit environments on fiber quality. Three EMS treated populations were advanced from the first to the fourth generation (M1 to M4) as bulk harvested populations. In 2014, single-plant divergent selection was applied based on perceived morphological and agronomic differences seen during and at the end of the season.

[Results] Analyses from these selections in 2014–2016 showed significant (P < 0.05) improvement between and within populations for fiber traits (micronaire, length, strength, uniformity, and elongation) when compared with the original non-treated EMS source; some selections were found to have excellent fiber quality under diverse irrigation-regimes.

[Conclusions] Some of these selections are being considered for germplasm release and could be useful for improving the fiber quality of cotton under water limited conditions, thereby helping to ensure the long-term survival of the cotton industry on the Texas High Plains.

Irrigation’s effect and applied selection on the fiber quality of Ethyl MethaneSulfonate (EMS) treated upland cotton (Gossypium hirsutum L.)
WITT Travis W. , ULLOA Mauricio, PELLETIER Mathew G. , MENDU Venugopal and RITCHIE Glen L.
Journal of Cotton Research. 2018; 1:17.
https://jcottonres.biomedcentral.com/articles/10.1186/s42397-018-0016-8

JCR-Cotton High Speed Phenotyping Thematic Series Call For Paper

Journal of Cotton Research

Cotton High Speed Phenotyping
Thematic Series Call For Paper
Coordinator: Professor Eric F. Hequet, Texas Tech University, USA; Dr. Glen Ritchie, Texas Tech University, USA

Author’s allowance: The sponsor, Institute of Cotton Research, CAAS, grants to cover not only APC for the submission, but also the author’s allowance once published.

High speed phenotyping is critical to improve cotton research and production. It can be applied to large scale commercial fields, research fields, breeding lines, and even at the individual plant level. The main goals are to improve yield, fiber quality, stress and disease resistance, etc… Recently, advances in high speed phenotyping in cotton have been achieved. The Journal of Cotton Research is hosting a thematic series on this topic. The research community is encouraged to share original findings, methodology, results, databases, and/or software and opinions.

Scopes that may be covered in the submissions may include, but are not limited to the following:
1. Platform design: air-based and/or land-based;
2. Data capture and processing: sensors (RGB, IR, multispectral, sonic, etc.), integration of multiple sensors, information processing technologies;
3. Data analysis and Metadata: analysis of very large data sets, validation with ground truth, practical application examples (breeding programs, site specific irrigation scheduling, etc.).

Submission Deadline: April 30, 2019

https://jcottonres.biomedcentral.com/cottonhsp

Map-based cloning of a recessive gene v1 for virescent leaf expression in cotton (Gossypium spp.)

[Background] Virescence, as a recognizable phenotype in the early development stage of cotton, is not only available for research on chloroplast development and photosynthesis but also for heterosis exploitation in cotton.

[Methods] In current study, for fine mapping of virescent-1 (v1) in cotton, three populations with a total of 5 678 individuals were constructed using T582 which has the virescent trait. Tobacco rattle virus, TRV1 and TRV2 (pYL156), were used as vectors for the virus-induced gene silencing (VIGS) assay.

[Results] The v1 gene was fine-mapped to a 20 kb interval on chromosome 20 of tetraploid cotton. We identified only one candidate gene with four single nucleotide polymorphisms between parents, among which the single nucleotide polymorphism at the position of 1 082 base pair caused the change of amino acid residue from Arg (3–79) to Lys (T582). The relative expression of the candidate gene in virescent plants was extensively lower than that in normal plants. Nullification of the gene by VIGS significantly turned the green leaf of normal cotton plants into yellow. We named this candidate gene as GhRVL.

[Conclusions] This study will facilitate the further research on virescent formation, and will be useful for breeding of hybrid cottons.

Map-based cloning of a recessive gene v1 for virescent leaf expression in cotton (Gossypium spp.)
ZHANG Youping, WANG Qiaolian, ZUO Dongyun, CHENG Hailiang, LIU Ke, ASHRAF Javaria, LI Simin, FENG Xiaoxu, YU John Z. and SONG Guoli
Journal of Cotton Research. 2018; 1:10.
https://doi.org/10.1186/s42397-018-0009-7

Identification and screening of nitrogen-efficient cotton genotypes under low and normal nitrogen environments at the seedling stage

[Background] Large quantities of nitrogen (N) fertilizer applied to cotton cropping systems support high yields but cause adverse environmental impacts such as N2O emission and water eutrophication. The development of cotton cultivars with higher N use efficiencies suitable for low-N conditions is therefore important for sustainable production. In this study, we evaluated 100 cotton genotypes in 2016 for N use efficiency and related traits at the seedling stage.

[Methods] Sand culture experiment was conducted with low N levels (0.01 g·kg−1) or normal N levels (0.1 g·kg−1). We investigated plant height, SPAD value (soil plant analysis development chlorophyll meter), dry weight, N accumulation, N utilization efficiency, and N uptake efficiency. Through descriptive statistics, principal component analysis and heatmap clustering analysis, we confirmed the evaluation index system of N-efficient genotypes and the classification of N-efficient genotypes.

[Results] Significant differences were observed among N levels and genotypes for all agronomic traits and N levels. Coefficients of variation varied greatly and ranged from 6.7~28.8 and 7.4~20.8 under low-N and normal-N treatment, respectively. All traits showed highly significant positive correlations with each other, except SPAD value. The principal components under both N levels were similar, showing that total dry weight, aboveground dry weight, total N accumulation, and N uptake efficiency were important components. We confirmed these four traits as suitable screening indexes for low N tolerance. Based on the results of heatmap clustering and scatter diagram analysis of N efficiency value, 10 genotypes were found low-N tolerant, in which five varieties were inefficient under both low and normal N conditions, while four varieties were found efficient under low-N conditions but inefficient under normal-N conditions. Only one variety was efficient under both low and normal-N conditions. Meanwhile, 20 genotypes were identified as low-N sensitive ones, in which 19 genotypes were inefficient under low-N conditions but efficient under normal-N conditions, one variety was inefficient under both low and normal-N conditions.

[Conclusion] We preliminarily identified Kashi as a low-N tolerant and N-efficient cotton genotype, and CCRI 64 as a low-N sensitive and N-inefficient cotton genotype. Further studies should be carried out to verify the yield and heritability effect of specific genotypes in the field.

Identification and screening of nitrogen-efficient cotton genotypes under low and normal nitrogen environments at the seedling stage
ZHANG Hengheng†, FU Xiaoqiong†, WANG Xiangru, GUI Huiping, DONG Qiang, PANG Nianchang, WANG Zhun, ZHANG Xiling and SONG Meizhen
†Contributed equally
Journal of Cotton Research. 2018; 1:6.
https://jcottonres.biomedcentral.com/articles/10.1186/s42397-018-0006-x

The current status of nitrogen fertiliser use efficiency and future research directions for the Australian cotton industry

Fifty years of sustained investment in research and development has left the Australian cotton industry well placed to manage nitrogen (N) fertiliser. The average production in the Australian cotton industry today is greater than two tonnes of lint per hectare due to improved plant genetics and crop management. However, this average yield is well below the yield that would be expected from the amount of N fertiliser used. It is clear from the recent studies that across all growing regions, conversion of fertiliser N into lint is not uniformly occurring at application rates greater than 200–240 kg·hm−2 of N. This indicates that factors other than N availability are limiting yield, and that the observed nitrogen fertiliser use efficiency (NFUE) values may be caused by subsoil constraints such as sodicity and compaction. There is a need to investigate the impact of subsoil constraints on yield and NFUE. Gains in NFUE will be made through improved N fertiliser application timing, better targeting the amount of fertiliser applied for the expected yield, and improved soil N management. There is also a need to improve the ability and confidence of growers to estimate the contribution of soil N mineralisation to the crop N budget. Many Australian studies including data that could theoretically be collated in a meta-analysis suggest relative NFUE values as a function of irrigation technique; however, with the extensive list of uncontrolled variables and few studies using non-furrow irrigation, this would be a poor substitute for a single field-based study directly measuring their efficacies. In irrigated cotton, a re-examination of optimal NFUE is due because of the availability of new varieties and the potential management and long-term soil resilience implications of the continued removal of mineralised soil N suggested by high NFUE values. NFUE critical limits still need to be derived for dryland systems.

The current status of nitrogen fertiliser use efficiency and future research directions for the Australian cotton industry
MACDONALD Ben C. T., LATIMER James O., SCHWENKE Graeme D., NACHIMUTHU Gunasekhar and BAIRD Jonathan C.
Journal of Cotton Research. 2018; 1:15.
https://doi.org/10.1186/s42397-018-0015-9

Designations for individual genomes and chromosomes in Gossypium

Gossypium, as the one of the biggest genera, the most diversity, and the highest economic value in field crops, is assuming an increasingly important role in studies on plant taxonomy, polyploidization, phylogeny, cytogenetics, and genomics. Here we update and provide a brief summary of the emerging picture of species relationships and diversification, and a set of the designations for individual genomes and chromosomes in Gossypium. This cytogenetic and genomic nomenclature will facilitate comparative studies worldwide, which range from basic taxonomic exploration to breeding and germplasm introgression.

Designations for individual genomes and chromosomes in Gossypium
WANG Kunbo, WENDEL Jonathan F. and HUA Jinping
Journal of Cotton Research. 2018; 1:3.
https://doi.org/10.1186/s42397-018-0002-1