Use of genetic and genomic approaches for combating cotton leaf curl disease

Back to Content

Authors

Rahman, Mehboob-ur-1; Ali, Ahmed1; Qaiser khan, Ali1; Abbas, Ammad1; Rahmat, Zainab1; Sarfraz, Zareen1; Khalid, Anum1; Gul, Maryam1; Munir, Atif1; Atifiqbal, Muhammad1; Scheffler, Jodi2; Scheffler, Brain2;

1National Institute for Biotechnology & Genetic Engineering (NIBGE) Faisalabad, Pakistan. 2Jamie Whitten Delta States Research Center, Stoneville, Mississippi, USA

Abstract

Cotton leaf curl, a disease of viral origin, is transmitted by a whitefly (Bemisiatabaci), was first reported in 1912 in Nigeria, and later spread to Egypt, Sudan, India and Pakistan, and recently in China. The disease has significantly challenged the sustainability of cotton production in Pakistan with annual yield penalty of two million bales. Efforts were made in developing resistant cotton varieties, which upheld the resistance for couple of years but overcame by the evolution of new strain of the virus (now called as cotton leaf curl Burewala virus). For protecting the most important natural fiber producing crop, a mega project aiming at the improvement of genetics of the cotton plant for combating the disease, was initiated in 2011 under Pak-US (managed through ICARDA, Pakistan) joint venture program. Till now, more than 3500 cotton accessions have been screened; and 33 accessions were found asymptomatic while G. hirsutum 2472-3 and G. hirsutum 3661 showed high tolerance to the disease. Among the asymptomatic, G. hirsutum Mac-07 (photoperiod insensitive) is being used extensively by multiple cotton research institutes for developing resistant cotton cultivars. A number of mapping populations by involving tolerant and resistant cotton genotypes with the mostsusceptible cotton species were developed. For example, mapping populations were developed by crossingG. hirsutum 2472-3 (tolerant), G. hirsutum Mac-07 (resistant) and highly sensitive genotypes G. barbadense PGMB-66, G. barbadense PIMA S7, respectively. A total of 1200 SSRs were surveyed on parent genotypes of G. hirsutum 2472-3 and G. barbadense PGMB-66 (Cross-I). Out of these, 113 were found polymorphic. These were surveyed on F2population. In second population (derived from a cross G. hir 2472-3/G. bar PIMA S-7, Cross-II), we surveyed 170 SSRs. Out of these, 24 polymorphic were surveyed on F2population. Similarly, we too surveyed 435 SSRs on the parent genotypes of third mapping population (Mac-7/PIMA S-7, Cross-III). A total of 18 polymorphic primers were surveyed on F2 population. Based on our limited studies, we were able to identify two QTLs i.e. QCLCuD25 and QCLCuD26 from Cross-I population study, six QTLs i.e. QCLCuD3, QCLCuD4, QCLCuD7, QCLCuD8, QCLCuD9, QCLCuD14 from Cross-II population study, and three QTLs from cross-III population. These studies will pave the way for not only initiating marker-assisted breeding for the development of resistant cotton cultivars in Pakistan but will also provide a comprehensive information to the international cotton community for combating the disease.

Back to Content

Effect of Plant Growth Promoting Rhizobacteria (PGPR) on Verticillium Wilt of Cotton

Back to Content

Authors

Hamdullah Korhan1, Lale Efe2, Yuksel Bolek3 and Mustafa Kusek4

1Kahramanmaras Sutcu ımam university, graduate school of natural and applied sciences, Kahramanmaras, Turkey

2Kahramanmaras Sutcu Imam University, Faculty of Agriculture, Department of

Field Crops, Kahramanmaras, Turkey,

3Kahramanmaras Sutcu Imam University Faculty of Agriculture, Department of Agricultural Biotechnology, Kahramanmaras, Turkey

4Kahramanmaras Sutcu Imam University Faculty of Agriculture, Department of

Plant Protection, Kahramanmaras, Turkey

Abstract

Verticillium wilt of cotton is a destructive disease that causes considerable yield losses on the crop. There is no effective method for controlling Verticillium wilt of cotton that is one of the world’s most devastating diseases except breeding resistant varieties. Due to environmental concerns of chemicals used in the control of this disease in which using antagonistic bacteria as a biocontrol agent to suppress the disease is very important. In addition, bacteria converting phosphorus to a form that is useful for the plant can offer an advantage for plant growth and disease resistance. Isolation the bacteria from the soil and use as plant growth regulation are the focus of some research has been going on.

Starting by 2014, this study will continue for 3 years. Starting, soil samples were collected at different vegetative periods of plant from 25 different cotton producing areas (from July to September) and pure strain cultivars were obtained from 650 soil samples. Tests applied to these strains are Hypersensitive Reaction on Tobacco, Levan Formation, Oxidase, Hydrogen Peroxide And Potassium Hydroxide. Resulting of identification tests, isolates that doesn’t have pathogenicity were tested for the ability to convert the phosphorus obtainable for by the plants, resulting of identification tests, isolates that doesn’t have pathogenicity will be used to antimicrobial activity in both greenhouse and in the field trials.

Back to Content

Leaf reddening index as an indicator of leaf reddening malady in bt cotton hybrids: causes and remedies

Back to Content

Authors

Y. Rakshitha1, B. S. Janagoudar* and A. Amaregouda2

1 Postgraduate scholar, 2 Professor of Crop Physiology

*        Correspondence : Dr. B. S. Janagoudar, Dean (Agri), College of Agriculture,

University of Agricultural Sciences, Dharwad – 580 005, Karnataka, India e-mail : bjanagoudar56@gmail.com, deanacd@uasd.in

Abstract

In the recent past, leaf reddening has been a major problem in Bt-cotton and this is an outcome of interaction of location, variety, environmental condition and nutrients. In general, inter and intra specific tetraploid Bt hybrids are more sensitive and vulnerable to this malady may be because of Bt gene interaction. Leaf reddening may occur at any growth stage of the crop. At grand growth  phase (flowering and boll development) any hindrance in the assimilate production, translocation and distribution intensifies the leaf reddening effect and symptoms are prolific in nature under extreme stress situations (Poongothai et  al., 2010).

Back to Content

JCR-Physiology and Pathology Thematic Series Call For Paper

Coordinator:
Professor Hezhong DONG, Cotton Research Centre, Shandong Academy of Agricultural Sciences, China;
Professor Zhiguo ZHOU, Nanjing Agricultural University, China

Journal of Cotton Research is hosting a thematic series on cotton physiology and pathology. The research community is encouraged to share original findings, methodology, results, databases, and/or software and opinions.

Scopes that may be covered in the submissions may include, but are not limited to the following:

1. growth and organ development;
2. plant and shoot architecture;
3. water, fertilizer, light, etc. use efficiency;
4. intelligent agriculture;
5. rhizosphere and other plant-environment interactions;
6. main disease and resistance.

Submission Deadline: 30 September 2019

Submit Now

https://jcottonres.biomedcentral.com/cottonpap

JCR-Cotton High Speed Phenotyping Thematic Series Call For Paper

Journal of Cotton Research

Cotton High Speed Phenotyping
Thematic Series Call For Paper
Coordinator: Professor Eric F. Hequet, Texas Tech University, USA; Dr. Glen Ritchie, Texas Tech University, USA

Author’s allowance: The sponsor, Institute of Cotton Research, CAAS, grants to cover not only APC for the submission, but also the author’s allowance once published.

High speed phenotyping is critical to improve cotton research and production. It can be applied to large scale commercial fields, research fields, breeding lines, and even at the individual plant level. The main goals are to improve yield, fiber quality, stress and disease resistance, etc… Recently, advances in high speed phenotyping in cotton have been achieved. The Journal of Cotton Research is hosting a thematic series on this topic. The research community is encouraged to share original findings, methodology, results, databases, and/or software and opinions.

Scopes that may be covered in the submissions may include, but are not limited to the following:
1. Platform design: air-based and/or land-based;
2. Data capture and processing: sensors (RGB, IR, multispectral, sonic, etc.), integration of multiple sensors, information processing technologies;
3. Data analysis and Metadata: analysis of very large data sets, validation with ground truth, practical application examples (breeding programs, site specific irrigation scheduling, etc.).

Submission Deadline: April 30, 2019

https://jcottonres.biomedcentral.com/cottonhsp

Seventh ‘Asian Cotton Research and Development Network’ Meeting, 15-17 Sept 2017, Nagpur India.

The Seventh Meeting of the Asian Cotton Research and Development Network was held at Nagpur during 15-17 September 2017. The Indian Society for Cotton Improvement (ISCI), Mumbai hosted the meeting together with the ICAR-CICR (Central Institute for Cotton Research), Nagpur and ICAR-CIRCOT (Central Institute for Research on Cotton Technologies). The main theme of the meeting was PRODUCING QUALITY FIBRE & DOUBLING COTTON FARMERS INCOME. Cotton researchers from public and the private sector participated. Dr. C. D. Mayee, President, Indian Society for Cotton Improvement, was the chairman, of the organizing Committee. The meeting elected Dr. Prashant G. Patil as Chairman of the Network until the next meeting. Eminent cotton experts i.e., Dr. Timorthy Dennehy, Dr Judith Brown, Dr Albert Santos from US; Dr Derek Russell from Australia, Dr Negm from Egypt and several senior research leaders from India and Bangladesh attended the meeting. The proceedings, recommendations, presentations and abstracts of the meeting can be accessed here.

Seventh Meeting of the ACRDN

Proceedings are available

The Seventh Meeting of the Asian Cotton Research and Development Network was held at Nagpur, India during 15-17 September 2017. The Indian Society for Cotton Improvement (ISCI), Mumbai hosted the meeting together with the ICAR-CICR (Central Institute for Cotton Research), Nagpur and ICAR-CIRCOT (Central Institute for Research on Cotton Technologies).

The main theme of the meeting was  “PRODUCING QUALITY FIBRE & DOUBLING COTTON FARMERS INCOME.” Cotton researchers from public and the private sector participated. Dr. C.D. Mayee, President, Indian Society for Cotton Improvement, was the Chairman of the Organizing Committee. The meeting elected Dr. Prashant G. Patil as Chairman of the Network until the next meeting. Eminent cotton experts like Dr. Timorthy Dennehy, Dr Judith Brown, Dr Albert Santos from US; Dr Derek Russell from Australia; Dr Negm from Egypt and several senior research leaders from India and Bangladesh attended the meeting.

The proceedings, recommendations, presentations and abstracts of the meeting are now available at the ICAC website.

Cotton Research enters to a period of “golden” opportunities

With the recent completion of draft sequencing of diploid cotton Gossypium raimondii and G. arboreum genomes published in Nature Genetics, and the first “gold-standard” version of G. raimondii genome published in Nature, cotton research community enjoyed the pick of many seminal research results that have provided a glorious opportunity to study orthologous and paralogous genes and gene families in allotetraploid cotton.

These successes and great achievements in ancestral diploid genome sequencing further resulted in decoding of the representative genome of widely grown allotetraploid Upland (G. hirsutum L.) cotton, Texas Marker-1.

Two independent research papers published in Nature Biotechnology by Zhang et al. and Li et al. in this week issue described the complex allotetraploid TM-1 genome that further entered cotton research to an era of “golden” opportunities providing the first insights into allotetraploid cotton genome structure, genome rearrangements, gene evolution, cotton fiber biology and biotechnology that will help to rapidly translate the genomics “knowledge” to an “economic impact”!

Congratulation to all cotton community with these latest achievements, high impact journal seminal publications, and hard work to foster cotton research!

World Cotton Germplasm Resources – new book has been published

New open access book of Intech entitled  “World Cotton Germplasm Resources” (ISBN 978-953-51-1622-6) has just been published online.  Book has compiled 11 peer-reviewed chapters from several leading cotton growing countries including Australia, China, India, Pakistan, United States of America, and Uzbekistan. It provided updated information on the current status and detailed inventory of available cotton germplasm resources. All chapters also targeted to address the past and current progress; enrichment of collections with novel germplasm resources including Bt-cotton, RNA interference and markers assisted selection lines; new trends and molecular tools in germplasm evaluations, development of database, understanding genetic diversity and its exploitation in cotton breeding; future perspectives of existing collections; critical challenges and opportunities in preserving the cotton genetic resources; and the ongoing multi-national communication and collaboration to enhance the germplasm protection, preservation, and evaluation. This book should be useful reading source on worldwide cotton germplasm resources. The content of this book is freely available for downloading at (http://www.intechopen.com/books/world-cotton-germplasm-resources).