Toward the efficient use of Beauveria bassiana in integrated cotton insect pest management

[Background] For controlling the resistance to insects, in particular carpophagous and phyllophagous caterpillars, using chemical pesticides has led to contamination of cotton area in Benin. Facing this problem, alternative methods including the use of entomopathogenic fungi as biopesticide could be a sound measure to preserve the environment, biodiversity and ensure good quality of crops. Previous studies have revealed the insecticidal potential of the entomopathogenic Beauveria bassiana on some insect pest species. However, little is known about its effectiveness on cotton Lepidopteran pests. This review is done to learn more about B. bassina for its application in controlling cotton insect pests, especially Lepidopteran species.

[Main body] Different sections of the current review deal with the related description and action modes of B. bassiana against insects, multi-trophic interactions between B. bassiana and plants, arthropods, soil and other microbes, and biological control programs including B. bassiana during last decade. Advantages and constraints in applying B. bassiana and challenges in commercialization of B. bassiana-based biopesticide have been addressed. In this review, emphasis is put on the application methods and targeted insects in various studies with regard to their applicability in cotton

[Conclusion] This review helps us to identify the knowledge gaps related to application of B. bassiana on cotton pest in general and especially in Lepidopteran species in Benin. This work should be supported by complementary laboratory bioassays, station and/or fields experiments for effective management of cotton Lepidopteran pests in Benin.
[Title] Toward the efficient use of Beauveria bassiana in integrated cotton insect pest management

[Authors] DANNON H. Fabrice, DANNON A. Elie, DOURO-KPINDOU O. Kobi, ZINSOU A. Valerien, HOUNDETE A. Thomas, TOFFA-MEHINTO Joëlle, ELEGBEDE I. A. T. Maurille, OLOU B. Dénis & TAMÒ Manuele

Journal of Cotton Research 2020, 324

https://doi.org/10.1186/s42397-020-00061-5

Heritability and correlation analysis of morphological and yield traits in genetically modified cotton

[Background] Cotton is known for fiber extraction and it is grown in tropical and sub-tropical areas of the world due to having hot weather. Cotton crop has a significant role in GDP of Pakistan. Therefore, the two-years research was conducted to estimate heritability and association among various yield contributing parameters of cotton, i.e., plant height, number of bolls per plant, number of sympodial branches per plant, seed cotton yield, boll weight, seed index, ginning outturn (GOT), fiber length, fiber strength, and fiber fineness.

[Result] Association analysis revealed that seed cotton yield had a significant positive correlation with plant height, number of bolls per plant, number of sympodial branches per plant, GOT, staple length and fiber strength. Staple length and fiber strength were negatively linked with each other. Estimates of heritability were high for all of the traits except the number of sympodial branches per plant and boll weight.

[Conclusion] The parent IUB-222 was found to be the best for plant height, the number of bolls per plant, boll weight, GOT, seed cotton yield, and seed index. The genotypes namely, NIAB-414 and VH-367 were identified as the best parents for fiber length, strength, and fineness. Among the crosses NIAB-414 × IUB-222 was the best for the number of bolls per plant, seed index, seed cotton yield and fiber fineness, whereas, the cross of NIAB-414 × CIM-632 was good for plant height. The combination of A555 × CIM-632 was the best for the number of sympodial branches per plant, boll weight, fiber length, and strength, and VH-367 × CIM-632 proved the best for GOT.
[Title] Heritability and correlation analysis of morphological and yield traits in genetically modified cotton

[Authors] REHMAN Abdul, MUSTAFA Nida, DU Xiongming & AZHAR Muhammad Tehsee

Journal of Cotton Research 2020, 323

https://doi.org/10.1186/s42397-020-00067-z

QTL and genetic analysis controlling fiber quality traits using paternal backcross population in upland cotton

[Background] Genetic improvement in fiber quality is one of the main challenges for cotton breeders. Quantitative trait loci (QTL) mapping provides a powerful approach to dissect the molecular mechanism in fiber quality traits. In present study, F14recombinant inbred line (RIL) population was backcrossed to paternal parent for a paternal backcross (BC/P) population, deriving from one upland cotton hybrid. Three repetitive BC/P field trials and one maternal backcross (BC/M) field trial were performed including both two BC populations and the original RIL population.

[Result] In total, 24 novel QTLs are detected for fiber quality traits and among which 13 QTLs validated previous results. Thirty-five QTLs in BC/P populations explain 5.01%–22.09% of phenotype variation (PV). Among the 35 QTLs, 23 QTLs are detected in BC/P population alone. Present study provides novel alleles of male parent for fiber quality traits with positive genetic effects. Particularly, qFS-Chr3–1 explains 22.09% of PV in BC/P population, which increaseds 0.48 cN·tex− 1 for fiber strength. A total of 7, 2, 8, 2 and 6 QTLs explain over 10.00% of PV for fiber length, fiber uniformity, fiber strength, fiber elongation and fiber micronaire, respectively. In RIL population, six common QTLs are detected in more than one environment: qFL-Chr1–2qFS-Chr5–1qFS-Chr9–1qFS-Chr21–1qFM-Chr9–1 and qFM-Chr9–2. Two common QTLs of qFE-Chr2–2(TMB2386-SWU12343) and qFM-Chr9–1 (NAU2873-CGR6771) explain 22.42% and 21.91% of PV. The region between NAU4034 and TMB1296 harbor 30 genes (379 kb) in A05 and 42 genes (49 kb) in D05 for fiber length along the QTL qFL-Chr5–1 in BC/P population, respectively. In addition, a total of 142 and 46 epistatic QTLs and QTL × environments (E-QTLs and QQEs) are identified in recombinant inbred lines in paternal backcross (RIL-P) and paternal backcross (BC/P) populations, respectively.

[Conclusion] The present studies provide informative basis for improving cotton fiber quality in different populations.
[Title] QTL and genetic analysis controlling fiber quality traits using paternal backcross population in upland cotton

[Authors] MA LingLing, SU Ying, NIE Hushuai, CUI Yupeng, CHENG Cheng, IJAZ Babar & HUA  Jinping

Journal of Cotton Research 2020, 322

https://doi.org/10.1186/s42397-020-00060-6

Overexpressing rice lesion simulating disease 1-like gene (OsLOL1) in Gossypium hirsutum promotes somatic embryogenesis and plant regeneration

[Background] Cotton somatic embryogenesis is difficult or rarely frequent to present, which has limited gene function identification and biotechnological utility. Here, we employed a rice key somatic embryogenesis-related gene, rice lesion simulating disease 1-like gene (OsLOL1), to develop transgenic cotton callus for evaluating its function in ectopic plants.

[Result] Overexpressing OsLOL1 can promote cotton callus to form embryogenic callus, not only shortening time but also increasing transition of somatic callus cells to embryogenic callus cells. And the regenerating plantlets per transgenic OsLOL1 embryogenic callus were significantly higher than those in the control transformed with empty vector. Analysis of physiological and biochemical showed that OsLOL1 can repress cotton superoxide dismutase 1 gene (GhSOD1) expression, possibly resulting in reactive oxidant species (ROS) accumulation in transgenic callus cells. And OsLOL1-overexpressed embryogenic callus exhibited higher α-amylase activity compared with the control, resulting from the promotion of OsLOL1 to cotton amylase 7 gene (GhAmy7) and GhAmy8 expression.

[Conclusion] The data showed that OsLOL1 could be used as a candidate gene to transform cotton to increase its somatic embryogenesis capacity, facilitating gene function analysis and molecular breeding in cotton.
[Title] Overexpressing rice lesion simulating disease 1-like gene (OsLOL1) in Gossypium hirsutum promotes somatic embryogenesis and plant regeneration

[Authors] WANG Zhian, WANG Peng, HU Guang, XIAO Juanli, ZHANG Anhong, LUO Xiaoli & WU Jiahe

Journal of Cotton Research 2020, 319

https://doi.org/10.1186/s42397-020-00062-4